There exceptions have been considered:
1. the file is empty
2.the size of genome is too small to suggest a primer of length 20
3.one genome A is subset of another genome B, there is no way to find a unique primer for genome A.
The program was tested to handle successfully these three exceptions.
test the program on the three strains of Bacillus anthracis:
Bacillus anthracis str.A0248.fa : ATAAGCGAATAAGAATGTAG
Bacillus anthracis str.CDC684.fa : TGAAGTTGCTGAGAAGAGAT
Bacillus anthracis str.Sterne.fa : CATCAGTTACAAAAAGAATG
real 168.97
user 167.91
sys 0.16
So far,the problems mentioned before have been solved. The next work is to write a scientific report for this project.
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